Premier League leaders Everton suffered their first defeat of the season as Southampton closed out an impressive 2-0 home win.
The Toffees boasted a 100 per cent record from their first four games and held defending champions Liverpool to a 2-2 draw in last week's Merseyside derby.
But Carlo Ancelotti's side left St Mary's with nothing on Sunday afternoon, conceding first-half goals to Saints skipper James Ward-Prowse and Che Adams and failing to score for the first time in the 2020-21 campaign.
Things ended on an even sourer note for Everton when Lucas Digne was shown a straight red card for a badly-timed challenge on Kyle Walker-Peters.
Leicester condemned Arsenal to their third defeat of the season as Jamie Vardy continued his hot streak against the Gunners.
With the score at 0-0 in the 80th minute the former England striker broke free, latching on to Cengiz Under's cross to nod a diving header past Bernd Leno.
The sides began the day locked in mid-table on nine points, with the result lifting the Foxes to fourth place while Mikel Arteta's men remained in 10th.
At Molineux a pair of late goals from Wolves and Newcastle livened up a tightly-fought encounter that ended 1-1.
The home side took the lead with 10 minutes remaining as Mexico striker Raul Jimenez lashed home a brilliant 20-yard effort after taking one touch to set himself.
Callum Wilson won a free-kick as the whistle approached and Jacob Murphy, on his first start of the season, cleverly whipped his effort around the wall to beat Rui Patricio at his near post.
